Which one of the following is a vital stain?

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(D) Vital stains are dyes used to stain living cells without killing them.$A$. Janus green-$B$ is specifically used to stain mitochondria in living ce

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All the above

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(D) Vital stains are dyes used to stain living cells without killing them.$A$. Janus green-$B$ is specifically used to stain mitochondria in living cells.$B$. Neutral red is a vital stain used to stain the nucleus and vacuoles in living cells.$C$. Methylene blue is commonly used as a vital stain for bacteria and various cellular components.Therefore,all the listed options are examples of vital stains.

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