Would you choose an exonuclease while produci | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(B) No,an exonuclease would not be chosen. Exonucleases act on the free ends of a linear $DNA$ molecule by removing nucleotides one by one. Instead of

Vedclass · Accepted Answer

No,because it degrades the $DNA$ from the ends.

Vedclass · Answer

(B) No,an exonuclease would not be chosen. Exonucleases act on the free ends of a linear $DNA$ molecule by removing nucleotides one by one. Instead of producing $DNA$ fragments with sticky ends suitable for ligation,an exonuclease would shorten or completely degrade the $DNA$ fragment containing the gene of interest. Furthermore,a circular plasmid (vector) lacks free ends,so an exonuclease would not be able to cut it at all. Therefore,restriction endonucleases are preferred as they cut $DNA$ at specific internal sites to produce sticky or blunt ends.

Would you choose an exonuclease while producing a recombinant $DNA$ molecule?

Similar Questions

What does the sample of the given base sequence represent?
$5'-GAATTC-3'$
$3'-CTTAAG-5'$

Identify true or false:
$(i)$ Restriction site is always a palindromic sequence.
$(ii)$ Palindromic sequence is always a restriction site.

$A$ plasmid without a selectable marker was chosen as a vector for cloning a gene. How does this affect the experiment?

Select the appropriate option regarding $r-DNA$.

In bacteria,a plasmid is .........

Vedclass Test Series

Exam Paper Generator

Online Exam Module