The ligation of an antibiotic resistance gene | vedclass

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(A) The enzyme $DNA$ ligase is responsible for joining $DNA$ fragments together by forming phosphodiester bonds between the $3'$-hydroxyl end of one n

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$DNA$ ligase

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(A) The enzyme $DNA$ ligase is responsible for joining $DNA$ fragments together by forming phosphodiester bonds between the $3'$-hydroxyl end of one nucleotide and the $5'$-phosphate end of another. In recombinant $DNA$ technology,$DNA$ ligase is used to ligate (join) the gene of interest (such as an antibiotic resistance gene) into the plasmid vector to create a recombinant $DNA$ molecule.

The ligation of an antibiotic resistance gene with a plasmid vector can be performed by which of the following enzymes?

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