In the context of blue-white screening,non-re | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(B) The blue-white screening method is based on the insertional inactivation of the $lacZ$ gene. $1$. The $lacZ$ gene encodes the enzyme $\beta$-galac

Vedclass · Accepted Answer

Non-recombinant bacteria contain beta-galactosidase.

Vedclass · Answer

(B) The blue-white screening method is based on the insertional inactivation of the $lacZ$ gene. $1$. The $lacZ$ gene encodes the enzyme $\beta$-galactosidase. $2$. In non-recombinant bacteria,the $lacZ$ gene is intact and functional,so the bacteria produce $\beta$-galactosidase. This enzyme reacts with the chromogenic substrate $X-gal$ to produce a blue color. $3$. In recombinant bacteria,the foreign $DNA$ is inserted into the $lacZ$ gene,causing insertional inactivation. Consequently,no functional $\beta$-galactosidase is produced,and the colonies remain white.

In the context of blue-white screening,non-recombinant bacterial colonies appear blue in contrast to white recombinant colonies because:

Similar Questions

The flowchart given below represents the process of recombinant $DNA$ technology. Identify $A$,$B$,and $C$.

In the presence of a chromogenic substrate,recombinant bacteria appear as .......

Assertion : The uptake of $DNA$ during transformation is an active,energy-requiring process.
Reason : Transformation occurs in only those bacteria,which possess the enzymatic machinery involved in the active uptake and recombination.

The $Polyethylene \text{ } Glycol$ $(PEG)$ method is used for:

Which of the following diagrams is correct for primer annealing in $PCR$?

Vedclass Test Series

Exam Paper Generator

Online Exam Module