In the presence of a chromogenic substrate,re | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(B) The process of insertional inactivation is used to distinguish recombinant from non-recombinant colonies. When the $lacZ$ gene is inserted with a

Vedclass · Accepted Answer

Colorless colonies

Vedclass · Answer

(B) The process of insertional inactivation is used to distinguish recombinant from non-recombinant colonies. When the $lacZ$ gene is inserted with a foreign $DNA$ sequence,the enzyme $\beta$-galactosidase is not produced. In the presence of a chromogenic substrate,non-recombinant bacteria produce blue-colored colonies because the $\beta$-galactosidase enzyme is functional. However,in recombinant bacteria,the $lacZ$ gene is inactivated,so the enzyme is not produced,and the colonies appear colorless (white).

In the presence of a chromogenic substrate,recombinant bacteria appear as .......

Similar Questions

$A$ mixture of fragmented $DNA$ was electrophoresed in an agarose gel. After staining the gel with ethidium bromide,no $DNA$ bands were observed. What could be the reason?

In a chemical process,the $DNA$ fragments produced by restriction endonucleases can be separated by:

$DNA$ fragments can be separated by using which of the following techniques?

Which of the following statements is incorrect?

After the biosynthetic phase, the product is separated & purified by the process called :-

Vedclass Test Series

Exam Paper Generator

Online Exam Module