Which of the following techniques is associated with the cloning method of tissue culture performed under in vitro conditions?

During $........$,scientists discovered that a whole plant could be regenerated from an explant.

At what speed is a rotary shaker continuously operated in suspension culture?

Which of the following is used in tissue culture?

Arrange the following steps of somatic hybridisation in a correct sequence.
$A$. Digestion of cell walls.
$B$. Isolation of naked protoplasts.
$C$. Fusion of protoplasts to get hybrid protoplast.
$D$. Isolation of single cells from two different varieties of plants.
$E$. Growing of hybrid protoplast to form a new plant.
Choose the correct answer from the options given below:

For which purpose are plants developed through the plant tissue culture method used?