$A$: $cDNA$ libraries are important to scientists in human genomics.
$R$: $cDNA$ is a synthetic type of $DNA$ generated from $mRNA$.

Assertion : $Agrobacterium$ $tumefaciens$ is popular in genetic engineering because this bacterium is associated with the roots of all cereal and pulse crops.
Reason : $A$ gene incorporated in the bacterial chromosomal genome gets automatically transferred to the crop with which the bacterium is associated.

Consider the following statements:
$I.$ Recombinant $DNA$ technology,popularly known as genetic engineering,is a stream of biotechnology which deals with the manipulation of genetic material by man in vitro.
$II.$ $pBR322$ is the first artificial cloning vector developed in $1977$ by Bolivar and Rodriguez from $E. coli$ plasmid.
$III.$ Restriction enzymes belong to a class of enzymes called nucleases.
Which of the statements given above are correct?

Match the following items in List-$I$ with List-$II$:

List-$I$	List-$II$
$(a)$ Gel electrophoresis	$(1)$ Amplification of gene of interest
$(b)$ $PCR$	$(2)$ Separation of $DNA$ fragments
$(c)$ Bioreactor	$(3)$ Plasmid
$(d)$ Cloning vector	$(4)$ Production of desired product

Which of the following statements is not true?
$I.$ $DNA$, being a hydrophilic molecule, cannot pass through cell membranes.
$II.$ $Agrobacterium$ $tumefaciens$ delivers a piece of $DNA$ known as $'T-DNA'$ in the $Ti$ plasmid, which transforms normal plant cells into tumour cells to produce chemicals against pathogens.
$III.$ Retrovirus, adenovirus, and papillomavirus are also now used as cloning vectors in animals because of their ability to transform normal cells into cancerous cells.
$IV.$ In genetic engineering, $DNA$ from different sources are cut with the same restriction enzymes so that both $DNA$ fragments have the same kind of sticky ends.
Choose the correct option.

Match the following:-

Column-$I$	Column-$II$
$(A)$ $\text{YAC}$	$(I)$ Separation of $\text{DNA}$ fragments
$(B)$ $\text{Hind II}$	$(II)$ Yeast
$(C)$ Gel electrophoresis	$(III)$ First restriction endonuclease
$(D)$ Heterologous host	$(IV)$ Recombinant protein