A : Ethidium bromide helps in visualizing DNA | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(A) Ethidium bromide $(EtBr)$ is a fluorescent dye used to stain $DNA$ fragments in agarose gel electrophoresis.When exposed to $UV$ light,the $DNA$ b

Vedclass · Accepted Answer

Assertion and Reason both are correct and Reason is the correct explanation of Assertion.

Vedclass · Answer

(A) Ethidium bromide $(EtBr)$ is a fluorescent dye used to stain $DNA$ fragments in agarose gel electrophoresis.When exposed to $UV$ light,the $DNA$ bands stained with $EtBr$ fluoresce as bright orange bands.This occurs because $EtBr$ is an intercalating agent that binds between the base pairs of the $DNA$ double helix.It absorbs light in the $UV$ range,specifically between $260-330 \; nm$,and emits light in the visible spectrum (orange).Therefore,both the Assertion and the Reason are correct,and the Reason provides the correct explanation for why $DNA$ can be visualized under $UV$ light.

$A$ : Ethidium bromide helps in visualizing $DNA$ in $UV$ light only.
$R$ : This intercalating agent absorbs light in range $260-330 \; nm$.

Similar Questions

In Recombinant $DNA$ technology,antibiotics are used:

What is the function of restriction endonucleases?

Recombinant $DNA$ can be obtained by cutting pro-$DNA$ using:

The part of the plasmid that codes for proteins involved in the replication of the $pBR322$ plasmid is:

Which technique is used to separate $DNA$ fragments?

Vedclass Test Series

Exam Paper Generator

Online Exam Module

$A$ : Ethidium bromide helps in visualizing $DNA$ in $UV$ light only.$R$ : This intercalating agent absorbs light in range $260-330 \; nm$.