Which technique is used to separate $DNA$ fragments?

$DNA$ from plant or animal tissues and bacterial cells can be obtained by treatment with appropriate enzymes. Match the following columns correctly.

Column-$I$	Column-$II$
$P$. Bacteria	$I$. Lysozyme
$Q$. Fungi	$II$. Cellulase
$R$. Plant cells	$III$. Chitinase

Given below are two statements.
Statement $I$: Restriction enzyme $BamHI$ has its recognition site in $tet^R$ region of $pBR322$.
Statement $II$: $E. coli$ having $pBR322$ with a desired $DNA$ if inserted at $BamHI$ site can grow in medium containing tetracycline.
In the light of the above statements, choose the correct answer from the options given below.

Manipulation of $DNA$ in genetic engineering became possible due to the discovery of

Which of the following statements does not hold true for restriction enzymes?

Select the appropriate option regarding $r-DNA$.