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(A) The isolation of restriction endonucleases by Nathans and Smith $(1970)$ made it possible to cut $DNA$ at specific sites.Restriction enzymes can c

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Restriction endonuclease

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(A) The isolation of restriction endonucleases by Nathans and Smith $(1970)$ made it possible to cut $DNA$ at specific sites.Restriction enzymes can cut both strands of $DNA$ when foreign nucleotides are introduced into the cell.They cleave $DNA$ to generate a nick with a $5'$ phosphoryl and $3'$ hydroxyl terminus,which is the fundamental step in recombinant $DNA$ technology.

Manipulation of $DNA$ in genetic engineering became possible due to the discovery of

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