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(D) The popularity and success of $PCR$ (polymerase chain reaction) are primarily due to the availability of thermostable $DNA$ polymerase.During the

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Availability of thermostable $DNA$ polymerase

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(D) The popularity and success of $PCR$ (polymerase chain reaction) are primarily due to the availability of thermostable $DNA$ polymerase.During the $PCR$ process,the reaction mixture is heated to high temperatures $(>90^{\circ} C)$ to denature the double-stranded $DNA$.Standard $DNA$ polymerases would denature and become inactive at these temperatures.Therefore,a thermostable $DNA$ polymerase,such as $Taq$ polymerase (isolated from the bacterium $Thermus$ $aquaticus$),is essential as it remains active even after repeated heating cycles.

Column-$I$	Column-$II$
$(A) E$	$(1) 1^{st}$ in order of identification
$(B) co$	$(2)$ Name of genus
$(C) R$	$(3)$ Name of species
$(D) I$	$(4)$ Name of strain

Which of the following has popularized the $PCR$ (polymerase chain reaction)?

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