What color does X-gal produce in the presence | vedclass

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(B) In recombinant $DNA$ technology,$X-gal$ ($5$-bromo$-4-$chloro$-3-$indolyl-$\beta$-$D$-galactopyranoside) is a chromogenic substrate used to detect

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(B) In recombinant $DNA$ technology,$X-gal$ ($5$-bromo$-4-$chloro$-3-$indolyl-$\beta$-$D$-galactopyranoside) is a chromogenic substrate used to detect the activity of the enzyme $\beta$-galactosidase.When the enzyme $\beta$-galactosidase is present and active,it cleaves the $X-gal$ molecule.This cleavage results in the formation of an insoluble blue-colored product.This process is commonly used in blue-white screening to identify recombinant colonies in cloning experiments.

Column-$I$	Column-$II$
$(a)$ Bioreactor	$(i)$ Separation of $DNA$ fragments
$(b)$ Electrophoresis	$(ii)$ Production of large quantities of products
$(c)$ $PCR$	$(iii)$ Detection of pathogen, based on antigen-antibody reaction
$(d)$ $ELISA$	$(iv)$ Amplification of nucleic acids

What color does $X-gal$ produce in the presence of $\beta$-galactosidase?

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