Mature insulin does not contain ........

The genetic defect-adenosine deaminase $(ADA)$ deficiency may be cured permanently by

$\text{ELISA}$ is :-

The name of the drug used in cancer treatment produced by biotechnology is:

Some of the steps involved in the production of humulin are given below. Choose the correct sequence.
$(i)$ Synthesis of gene $(DNA)$ for human insulin artificially.
(ii) Culturing recombinant $E. coli$ in bioreactors.
(iii) Purification of humulin.
(iv) Insertion of human insulin gene into plasmid.
$(v)$ Introduction of recombinant plasmid into $E. coli$.
(vi) Extraction of recombinant gene product from $E. coli$.

How many of the following statements are incorrect?
$I.$ Genes of Agrobacterium are used to produce humulin.
$II.$ Indian government has set up $\text{GEAC}$.
$III.$ $\alpha-1$-antitrypsin is used to treat $\text{PKU}$.
$IV.$ $\text{ELISA}$ serves the purpose of early diagnosis.
$V.$ Flavr-savr is a variety of pomato.