Select the correct sequence of steps in PCR. | vedclass

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(C) The $PCR$ (Polymerase Chain Reaction) process consists of three main steps:$1$. $Denaturation$: The double-stranded $DNA$ is heated to a high temp

Vedclass · Accepted Answer

$(i) 	ext{ Denaturation } (ii) 	ext{ Annealing } (iii) 	ext{ Extension}$

Vedclass · Answer

(C) The $PCR$ (Polymerase Chain Reaction) process consists of three main steps:$1$. $Denaturation$: The double-stranded $DNA$ is heated to a high temperature (approx. $94-98^{\circ}C$) to separate the strands.$2$. $Annealing$: The temperature is lowered (approx. $50-65^{\circ}C$) to allow primers to bind to the single-stranded $DNA$ templates.$3$. $Extension$: The temperature is adjusted (approx. $72^{\circ}C$) for the $Taq$ polymerase enzyme to synthesize the new $DNA$ strand by adding nucleotides.Therefore, the correct sequence is $Denaturation \rightarrow Annealing \rightarrow Extension$.

Select the correct sequence of steps in $PCR$.

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