In which method is breeding carried out between a male and a female of two different species?

Read the following four statements $(1-4)$ and identify the two statements that contain mistakes:
$1.$ The first transgenic buffalo,Rosie,produced milk which was human alpha-lactalbumin enriched.
$2.$ Restriction enzymes are used in the isolation of $DNA$ from other macromolecules.
$3.$ Downstream processing is one of the steps of $R-DNA$ technology.
$4.$ Disarmed pathogen vectors are also used in the transfer of $R-DNA$ into the host.
Which are the two statements having mistakes?

Match List-$I$ with List-$II$.

List-$I$	List-$II$
$(a)$ Protoplast fusion	$(i)$ Totipotency
$(b)$ Plant tissue culture	$(ii)$ Pomato
$(c)$ Meristem culture	$(iii)$ Somaclones
$(d)$ Micropropagation	$(iv)$ Virus-free plants

Choose the correct answer from the options given below.

The transfer of the streptomycin resistance gene from $Nicotiana$ $tabacum$ to $Nicotiana$ $sylvestris$ was achieved through which method?

Which of the following is/are considered as application$(s)$ of biotechnology?
$I$. Waste treatment
$II$. Energy production
$III$. Bioremediation
$IV$. Processed food
$V$. Genetically modified crops for agriculture
$VI$. Diagnostics
$VII$. Therapeutics
Choose the correct option.

Match the following columns:

Column-$I$	Column-$II$
$(a)$ Pest resistant plant	$(1)$ Transgenic cow
$(b)$ Golden rice	$(2)$ $C$-peptide
$(c)$ Proinsulin	$(3)$ High vitamin $A$ content
$(d)$ Rosie	$(4)$ $RNA$ interference