Amino acids contain which of the following functional groups?

What is the total number of essential amino acids?

How many amino acids are linked together by $(n-1)$ amide bonds?

Assertion: $\alpha$-amino acids exist as internal salt in solution as they have amino and carboxylic acid groups in near vicinity.
Reason: $H^{+}$ ion given by carboxylic group $(-COOH)$ is captured by amino group $(-NH_2)$ having lone pair of electrons.

The $pK_a$ values for the three ionizable groups $X, Y$ and $Z$ of glutamic acid are $4.3, 9.7$ and $2.2$ respectively. The structure is $HO_2C-CH_2-CH_2-CH(NH_3^+)-CO_2H$. The isoelectric point $(pI)$ for this amino acid is:

Sanger's reagent is used for the identification of which of the following?