Bacterial colonies that have a foreign text{D | vedclass

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(B) In the process of recombinant $	ext{DNA}$ technology,the $Lac \ Z$ gene encodes for the enzyme $\beta-$galactosidase. When a foreign $	ext{DNA}$

Vedclass · Accepted Answer

$\beta-$galactosidase gene shows insertional inactivation due to insertion of foreign $	ext{DNA}$

Vedclass · Answer

(B) In the process of recombinant $	ext{DNA}$ technology,the $Lac \ Z$ gene encodes for the enzyme $\beta-$galactosidase. When a foreign $	ext{DNA}$ fragment is inserted into this gene,it results in the disruption of the gene,a process known as insertional inactivation. As a result,the enzyme $\beta-$galactosidase is not produced. In the presence of a chromogenic substrate like $X-$gal,non-recombinant colonies (where the gene is intact) produce the enzyme,which cleaves $X-$gal to produce blue-colored colonies. Conversely,recombinant colonies (where the gene is inactivated) cannot produce the enzyme,so they cannot cleave $X-$gal and thus appear white.

Column-$I$	Column-$II$
$(a)$ Lysozyme	$(i)$ Fat
$(b)$ Cellulase	$(ii)$ Protein
$(c)$ Chitinase	$(iii)$ Bacteria
$(d)$ Lipase	$(iv)$ Fungi (Penicillium)
	$(v)$ Plant (Mangifera indica)

Bacterial colonies that have a foreign $\text{DNA}$ fragment inserted into the plasmid will appear white,because $-$

Similar Questions

Plasmid $pBR322$ has a $PstI$ restriction enzyme site within the gene $amp^R$ that confers ampicillin resistance. If this enzyme is used for inserting a gene for $\beta$-galactosidase production and the recombinant plasmid is inserted into an $E. coli$ strain:

Match the columns:

Column-$I$ Column-$II$

$(a)$ Lysozyme $(i)$ Fat

$(b)$ Cellulase $(ii)$ Protein

$(c)$ Chitinase $(iii)$ Bacteria

$(d)$ Lipase $(iv)$ Fungi (Penicillium)

$(v)$ Plant (Mangifera indica)

How can a recombinant protein be obtained?

Identify the correct sequence of steps in the Polymerase Chain Reaction $(PCR)$.

Polymerase chain reaction is most useful in

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