Plasmid pBR322 has a PstI restriction enzyme | vedclass

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(A) The plasmid $pBR322$ contains two antibiotic resistance genes: $amp^R$ (ampicillin resistance) and $tet^R$ (tetracycline resistance).Insertion of

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it will not be able to confer ampicillin resistance to the host cell.

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(A) The plasmid $pBR322$ contains two antibiotic resistance genes: $amp^R$ (ampicillin resistance) and $tet^R$ (tetracycline resistance).Insertion of a foreign $DNA$ fragment at the $PstI$ restriction site,which is located within the $amp^R$ gene,results in insertional inactivation of the $amp^R$ gene.Due to this inactivation,the recombinant plasmid loses its ability to confer ampicillin resistance to the host $E. coli$ cell.Therefore,the transformed cells will be sensitive to ampicillin but will be able to express the inserted gene for $\beta$-galactosidase.

Plasmid $pBR322$ has a $PstI$ restriction enzyme site within the gene $amp^R$ that confers ampicillin resistance. If this enzyme is used for inserting a gene for $\beta$-galactosidase production and the recombinant plasmid is inserted into an $E. coli$ strain:

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