Insertion of a foreign DNA at BamHI site in a | vedclass

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(C) The cloning vector $pBR322$ contains two antibiotic resistance genes: one for ampicillin $(amp^R)$ and one for tetracycline $(tet^R)$.In $pBR322$,

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$C$. Tetracycline

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(C) The cloning vector $pBR322$ contains two antibiotic resistance genes: one for ampicillin $(amp^R)$ and one for tetracycline $(tet^R)$.In $pBR322$, the $BamHI$ restriction site is specifically located within the tetracycline resistance $(tet^R)$ gene.When a foreign $DNA$ fragment is inserted at the $BamHI$ site, it disrupts the coding sequence of the $tet^R$ gene.As a result, the bacteria containing this recombinant plasmid lose their ability to survive in the presence of tetracycline, a phenomenon known as insertional inactivation.

Insertion of a foreign $DNA$ at $BamHI$ site in an $E. coli$ cloning vector $pBR322$ results in the loss of antibiotic resistance towards :

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