Which of the following range of temperature is required for denaturation,annealing of primer,and primer extension of $PCR$ respectively?

  • A
    $90-98^{\circ} C, 40-60^{\circ} C, 70-75^{\circ} C$
  • B
    $70-75^{\circ} C, 40-60^{\circ} C, 90-98^{\circ} C$
  • C
    $90-98^{\circ} C, 70-75^{\circ} C, 40-60^{\circ} C$
  • D
    $40-60^{\circ} C, 90-98^{\circ} C, 70-75^{\circ} C$

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