Which of the following represents a correct palindromic sequence recognized by $EcoRI$?

Which one is not a restriction enzyme?

Which of the following statements is not correct regarding the $Eco RI$ restriction endonuclease enzyme?
$I.$ $Eco RI$ restriction endonuclease enzyme is isolated from $Escherichia \; coli \; RY13$.
$II.$ Its recognition sequence is $5^{\prime}-GAATTC-3^{\prime}$ / $3^{\prime}-CTTAAG-5^{\prime}$.
$III.$ Its site of cleavage is between $G$ and $A$ in the sequence $5^{\prime}-GAATTC-3^{\prime}$.

In gel electrophoresis, the separated $DNA$ fragments can be visualized only after staining the $DNA$ with a compound known as ............. followed by exposure to $UV$ radiation.

Which of the following is used in recombinant $DNA$ technique?

Match the following columns:

Column-$I$	Column-$II$
$(a) \ Cla \ I$	$(1) \ \text{Antibiotic resistance gene}$
$(b) \ pBR322$	$(2) \ \text{Origin of replication}$
$(c) \ tet^R$	$(3) \ \text{Restriction enzyme}$
$(d) \ ori$	$(4) \ \text{Plasmid}$