(D) In gel electrophoresis, $DNA$ fragments are separated based on their size through a gel matrix (usually agarose). Since $DNA$ is colorless, it can

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(D) In gel electrophoresis, $DNA$ fragments are separated based on their size through a gel matrix (usually agarose). Since $DNA$ is colorless, it cannot be seen directly. To visualize the separated $DNA$ fragments, the gel is stained with a fluorescent dye called $Ethidium bromide$ $(EtBr)$. After staining, when the gel is exposed to $UV$ radiation, the $DNA$ bands appear as bright orange-colored bands.

Class 12 Biology Biotechnology Principals and Process Tools of recombinant DNA technology

Easy

In gel electrophoresis, the separated $DNA$ fragments can be visualized only after staining the $DNA$ with a compound known as ............. followed by exposure to $UV$ radiation.

A
Cobalt bromide
B
Sodium bromide
C
Magnesium bromide
D
Ethidium bromide

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Class 12 Questions

Class 12

Biology Questions

Chapter

Biotechnology Principals and Process

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Tools of recombinant DNA technology

What modification is done on the $Ti$ plasmid of Agrobacterium tumefaciens to convert it into a cloning vector?

Medium

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Assertion : Restriction enzymes cut the strand of $DNA$ to produce sticky ends.
Reason : Stickiness of the ends facilitates the action of the enzyme $DNA$ polymerase.

MediumAIIMS 2009

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Discuss with your teacher and find out how to distinguish between (Any two):
$(a)$ Plasmid $DNA$ and Chromosomal $DNA$
$(b)$ $RNA$ and $DNA$
$(c)$ Exonuclease and Endonuclease

Medium

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Ligation of foreign $\text{DNA}$ at which of the following site will result in loss of tetracycline resistance of $\text{pBR}^{322}$?

Easy

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$Taq$ polymerase is isolated from a

Medium

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In gel electrophoresis, the separated $DNA$ fragments can be visualized only after staining the $DNA$ with a compound known as ............. followed by exposure to $UV$ radiation.

Similar Questions

What modification is done on the $Ti$ plasmid of Agrobacterium tumefaciens to convert it into a cloning vector?

Assertion : Restriction enzymes cut the strand of $DNA$ to produce sticky ends.Reason : Stickiness of the ends facilitates the action of the enzyme $DNA$ polymerase.

Discuss with your teacher and find out how to distinguish between (Any two):$(a)$ Plasmid $DNA$ and Chromosomal $DNA$$(b)$ $RNA$ and $DNA$$(c)$ Exonuclease and Endonuclease

Ligation of foreign $\text{DNA}$ at which of the following site will result in loss of tetracycline resistance of $\text{pBR}^{322}$?

$Taq$ polymerase is isolated from a

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Assertion : Restriction enzymes cut the strand of $DNA$ to produce sticky ends.
Reason : Stickiness of the ends facilitates the action of the enzyme $DNA$ polymerase.

Discuss with your teacher and find out how to distinguish between (Any two):
$(a)$ Plasmid $DNA$ and Chromosomal $DNA$
$(b)$ $RNA$ and $DNA$
$(c)$ Exonuclease and Endonuclease