Which one of the following techniques is not | vedclass

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(C) In Thin Layer Chromatography $(TLC)$,the separated components are often invisible to the naked eye. To visualize them,several techniques are used:

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Visualisation agent as a component of mobile phase

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(C) In Thin Layer Chromatography $(TLC)$,the separated components are often invisible to the naked eye. To visualize them,several techniques are used: $1$. $I_{2}$ (Solid) chambers: Iodine vapors adsorb onto the components,making them visible. $2$. $U.V.$ Light: Many organic compounds fluoresce under $U.V.$ light. $3$. Spraying of an appropriate reagent: Chemical reagents react with the components to produce colored spots. However,adding a visualization agent directly to the mobile phase is not a standard technique for spotting components on a $TLC$ plate,as it would interfere with the separation process itself.

List-$I$ $(\text{Mixture})$	List-$II$ $(\text{Method of Separation})$
$A. CHCl_3 + C_6H_5NH_2$	$I. \text{Distillation under reduced pressure}$
$B. \text{Crude oil in petroleum industry}$	$II. \text{Steam distillation}$
$C. \text{Glycerol from spent-lye}$	$III. \text{Fractional distillation}$
$D. \text{Aniline-water}$	$IV. \text{Simple distillation}$

Which one of the following techniques is not used to spot components of a mixture separated on a thin layer chromatographic plate?

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