Which restriction site is present in the $tet^{R}$ gene in $pBR322$?

What was linked with the plasmid in the construction of the first $r-DNA$?

The discovery of which of the following has made it possible to cut $DNA$ at specific locations?

$A$ single-stranded piece of nucleic acid tagged with a radioactive molecule,which is used to detect a specific gene through hybridization,is called:

Create a chart (with diagrammatic representation) showing a restriction enzyme,the substrate $DNA$ on which it acts,the site at which it cuts $DNA$,and the product it produces.

Polyethylene glycol method is used for