Hybridoma cells are .....

In $1983$, Eli Lilly, an American company, prepared two $DNA$ sequences corresponding to $A$ and $B$ chains of human insulin and introduced them in plasmids of which organism to produce insulin chains?

Which one of the following is now being commercially produced by biotechnological procedures?

Match List-$I$ with List-$II$:

List-$I$	List-$II$
$A$. Genetically engineered Human Insulin	$I$. Gene therapy
$B$. $\text{GM}$ Cotton	$II$. $\text{E. coli}$
$C$. $\text{ADA}$ Deficiency	$III$. Antigen-antibody interaction
$D$. $\text{ELISA}$	$IV$. $\text{Bacillus thuringiensis}$

Choose the correct answer from the options given below:

Which vaccine is produced using yeast?

Which of the following techniques serves the purpose of early diagnosis of $AIDS$,cancer,etc?
$I$. Polymerase chain reaction
$II$. Recombinant $DNA$ technology
$III$. Enzyme-linked immunosorbent assay
Choose the correct option.