Which restriction enzymes have recognition si | vedclass

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(C) The plasmid $pBR322$ is a widely used cloning vector in biotechnology.It contains two antibiotic resistance genes: the ampicillin resistance gene

Vedclass · Accepted Answer

$Pvu \,I$ and $Pst \,I$

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(C) The plasmid $pBR322$ is a widely used cloning vector in biotechnology.It contains two antibiotic resistance genes: the ampicillin resistance gene $(amp^R)$ and the tetracycline resistance gene $(tet^R)$.The ampicillin resistance gene $(amp^R)$ contains unique recognition sites for the restriction enzymes $Pvu \,I$ and $Pst \,I$.The tetracycline resistance gene $(tet^R)$ contains recognition sites for $BamH \,I$ and $Sal \,I$.Therefore,the correct option is $Pvu \,I$ and $Pst \,I$.

Which restriction enzymes have recognition sites within the ampicillin resistance gene of $pBR322$?

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