What type of vaccine is the Hepatitis $B$ vaccine?

Following enzymes/chemical/technique are used in the process of gel electrophoresis:
$I.$ sample $DNA$ is cut into fragments
$II.$ restriction endonucleases
$III.$ agarose gel
$IV.$ ethidium bromide
$V.$ $UV$-radiation
$VI.$ elution
Mark the correct sequence of their use.

Select the option that represents the correct sequence of steps in $r-DNA$ technology.
$(1)$ Insertion of $r-DNA$ into the host
$(2)$ Isolation of $DNA$
$(3)$ Ligation of $DNA$ fragment into the vector
$(4)$ Culturing the host cells in a medium at a large scale and extraction of the desired product
$(5)$ Isolation of the desired $DNA$ fragment
$(6)$ Fragmentation of $DNA$ by restriction endonucleases

Which of the following processes is $NOT$ a part of the downstream processing?

Production of a human protein in bacteria by genetic engineering is possible because

The master plate in the replica plating experiment contained the: