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(B) The process of gel electrophoresis involves the following steps in sequence:$1$. First,the sample $DNA$ is cut into fragments using restriction en

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$I, II, III, IV, V, VI$

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(B) The process of gel electrophoresis involves the following steps in sequence:$1$. First,the sample $DNA$ is cut into fragments using restriction endonucleases ($I$ and $II$).$2$. These fragments are then separated based on their size by running them through an agarose gel $(III)$.$3$. To visualize the separated $DNA$ fragments,the gel is stained with ethidium bromide $(IV)$.$4$. The stained $DNA$ bands are then observed under $UV$-radiation $(V)$.$5$. Finally,the desired $DNA$ bands are cut out from the agarose gel and extracted,a process known as elution $(VI)$.Therefore,the correct sequence is $I, II, III, IV, V, VI$.

Column-$I$	Column-$II$
$(a)$ Lysozyme	$(i)$ Fat
$(b)$ Cellulase	$(ii)$ Protein
$(c)$ Chitinase	$(iii)$ Bacteria
$(d)$ Lipase	$(iv)$ Fungi (Penicillium)
	$(v)$ Plant (Mangifera indica)

Following enzymes/chemical/technique are used in the process of gel electrophoresis:
$I.$ sample $DNA$ is cut into fragments
$II.$ restriction endonucleases
$III.$ agarose gel
$IV.$ ethidium bromide
$V.$ $UV$-radiation
$VI.$ elution
Mark the correct sequence of their use.

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Match the columns:

Column-$I$ Column-$II$

$(a)$ Lysozyme $(i)$ Fat

$(b)$ Cellulase $(ii)$ Protein

$(c)$ Chitinase $(iii)$ Bacteria

$(d)$ Lipase $(iv)$ Fungi (Penicillium)

$(v)$ Plant (Mangifera indica)

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Following enzymes/chemical/technique are used in the process of gel electrophoresis:$I.$ sample $DNA$ is cut into fragments$II.$ restriction endonucleases$III.$ agarose gel$IV.$ ethidium bromide$V.$ $UV$-radiation$VI.$ elutionMark the correct sequence of their use.