| $PCR$ | Plasmid |
|---|---|
| $(1)$ It is an abiotic method of $DNA$ amplification. | $(1)$ It is a biotic vector molecule used for cloning. |
| $(2)$ $PCR$ can generate millions of copies of specific $DNA$ fragments in a short time. | $(2)$ Being a biotic system,the number of copies obtained is generally lower and depends on the host cell replication. |
| $(3)$ $PCR$ is an in vitro process used to amplify specific $DNA$ sequences. | $(3)$ Plasmids are used as vectors to introduce and replicate desired $DNA$ fragments within a living host cell. |
| $(4)$ The process involves cyclic steps: denaturation,annealing,and extension. | $(4)$ Plasmid-based cloning involves restriction digestion,ligation,and transformation,not cyclic $PCR$ steps. |
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