Which of the following tools of recombinant t | vedclass

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(B) The correct answer is $B$. $	ext{DNA}$ ligase is an enzyme that acts as a 'molecular glue' to join two $	ext{DNA}$ fragments by forming phosphod

Vedclass · Accepted Answer

$	ext{DNA}$ ligase $-$ Creates sticky ends of restriction fragments

Vedclass · Answer

(B) The correct answer is $B$. $	ext{DNA}$ ligase is an enzyme that acts as a 'molecular glue' to join two $	ext{DNA}$ fragments by forming phosphodiester bonds between them. It does not create sticky ends; rather,it joins fragments that often already have complementary sticky ends or blunt ends. Restriction enzymes are responsible for creating sticky or blunt ends by cutting $	ext{DNA}$ at specific recognition sequences. Therefore,the pairing in option $B$ is incorrect.

Which of the following tools of recombinant $\text{DNA}$ technology is incorrectly paired with its use?

Similar Questions

Assertion : Restriction enzymes cut the strand of $DNA$ to produce sticky ends.
Reason : Stickiness of the ends facilitates the action of the enzyme $DNA$ polymerase.

Which bond is formed by $DNA$ ligase to join a plasmid and a foreign $DNA$ fragment?

The most commonly used enzyme for polymerase chain reaction is

What is used as a medium in gel electrophoresis?

Define the following terms: Sticky ends and Transgene.

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