Which one of the following is regarded as a natural genetic engineer?

  • A
    Klebsiella frosteri
  • B
    Bacillus subtilis
  • C
    Agrobacterium tumefaciens
  • D
    Neurospora

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The role of $DNA$ ligase in the construction of a recombinant $DNA$ molecule is:

$A$ : Ethidium bromide helps in visualizing $DNA$ in $UV$ light only.
$R$ : This intercalating agent absorbs light in range $260-330 \; nm$.

$K_{12}$ plasmid was studied first in

Assertion : Restriction enzymes cut the strand of $DNA$ to produce sticky ends.
Reason : Stickiness of the ends facilitates the action of the enzyme $DNA$ polymerase.

While isolating $DNA$ from bacteria,which of the following enzymes is not used?

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